منابع مشابه
Expansion microscopy
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. Here we report the discovery that, by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced clo...
متن کاملExpansion Microscopy : Improving Imaging Through Uniform Tissue Expansion
Until the past decade, optical microscopy of biological specimens was strongly limited by diffraction and scattering, affecting imaging resolution and depth, respectively. Now, numerous methods are available to overcome each of these limitations, but sub-diffraction limited resolution imaging over large volumes of scattering tissue is still a challenge. This work concerns the development of a n...
متن کاملAlveolar expansion imaged by optical sectioning microscopy.
During lung expansion, the pattern of alveolar perimeter distension is likely to be an important determinant of lung functions as, for example, surfactant secretion. However, the segmental characteristics of alveolar perimeter distension remain unknown. Here, we applied real-time confocal microscopy in the isolated, perfused rat lung to determine the micromechanics of alveolar perimeter distens...
متن کاملExpansion microscopy: development and neuroscience applications.
Many neuroscience questions center around understanding how the molecules and wiring in neural circuits mechanistically yield behavioral functions, or go awry in disease states. However, mapping the molecules and wiring of neurons across the large scales of neural circuits has posed a great challenge. We recently developed expansion microscopy (ExM), a process in which we physically magnify bio...
متن کاملExpansion microscopy of zebrafish for neuroscience and developmental biology studies
Expansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility of ExM in the larval and embryonic zebrafish, an important model organism for the study of neuroscience and development. Regarding neuroscience, we found that ExM enabled the tracing of fine processes of radial gl...
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ژورنال
عنوان ژورنال: Current Protocols in Cytometry
سال: 2019
ISSN: 1934-9297,1934-9300
DOI: 10.1002/cpcy.67